recombinant human rh cntf  (R&D Systems)

Journal: The Journal of endocrinology

Article Title: Effects of the gp130 cytokines ciliary neurotropic factor (CNTF) and interleukin-11 on pituitary cells: CNTF receptors on human pituitary adenomas and stimulation of prolactin and GH secretion in normal rat anterior pituitary aggregate cultures.

doi: 10.1677/joe.0.1690539

Figure Lengend Snippet: Figure 3 Effect of rhIL-11 or rhCNTF on GH secretion in somatotropic and prolactinoma human pituitary adenoma cells in monolayer cultures. Cells were seeded at 2105 cells/well in 24-well plates. After serum depletion for 24 h, treatments with rhIL-11 or rhCNTF for 24 h were performed in fresh serum- depleted medium. (A) After 24 h of CNTF treatment (1·2 nM) the supernatants were collected and PRL was measured by IRMA. Values represent the meansS.E.M. of one of two separate adenomas, with four wells per treatment group. *P<0·05 compared with basal (ANOVA with Scheffé’s test). (B) After 24 h treatment with rhIL-11 (5 nM) or rhCNTF (2 nM), the supernatants were collected and GH was measured by RIA. Values represent the meansS.E.M. of one of eight separate adenomas, with four wells per treatment group. The other experiments gave comparable results. There were no significant difference between treatments (ANOVA with Scheffé’s test). Other doses of IL-11 (1·25 and 2·5 nM) and CNTF (0·4 and 1·2 nM) that were also tested did not show any effect. Data from another two tumors with higher basal values and similar results are not shown.

Article Snippet: In the light of our previous experience in studies of the effects of interleukin (Arzt et al. 1999, Perez Castro et al. 2000), recombinant rat (rr) and human (rh) CNTF (R&D-Systems Inc., Minneapolis, MN, USA), and rhIL-11 (Roche Molecular Biochemicals, Mannheim, Germany) were dissolved in 0·05% BSA (crystallized and lyophilized, 99% purity) and used at the indicated doses, the same diluent was used as control in all experiments referred to as ‘basal’.

Techniques: Serum Depletion